Estimation of Lupeol and Diosgenin Biomarker in “Mutrakrichantak Churna’ – An Ayurvedic Poly Herbal Formulation by UV & HPLC

Abstract

Mutrakrichantak churna (MC) is a polyherbal ayurvedic medicine, used for the treatment of various urinary disorders such as renal calculi, nephritits. The main objective of the study is validation and quantitative estimation of Lupeol and Diosgenin in marketed Mutrakrichantak churna was performed by UV and HPLC method. This study focuses on the estimation and quantification of key marker compounds present in the formulation using UV-visible spectroscopy and High-Performance Liquid Chromatography (HPLC) techniques. Marker compounds such as Lupeol and Diosgenin were identified and quantified. Standardization of herbal formulations ensures batch-to-batch consistency, safety, and efficacy. UV analysis was performed for rapid detection, while HPLC provided precise quantification. The methods were validated according to ICH guidelines with parameters including linearity, precision, accuracy, and LOD/LOQ. The study demonstrated that the analytical methods employed were accurate, reliable, and suitable for routine quality control of Mutrakrichantak Churna.