Amylase and Trypsin Activity in an Economically Important Fish Labeo Calbasu of Tripura, India

Abstract

Labeo calbasu is considered as one of the preferred fish in the market of different corners of India. Certain biochemistry profile of this fish species at its different life cycle stages  has been analysed. The digestive enzyme activity shows the effective role of amylase (936.00-1014.00 in activity unit per 100 g fish body weight) and trypsin (44.00-62.00 in activity unit per 100 g fish body weight) in Labeo calbasu.  With the examination of faecal matter from its stomach, fore-gut, hind-gut and anus area of the fish sample it depicts variation of apparent digestibility coefficient in its pre-adult as well as adult stage.  Pre-adult stage exhibits excretion of 20% protein in the faecal matter while adult stage showing excretion of 40% protein in the faeces. Metabolic faecal protein during the adult stage of the fish amounting 3.6 g/100g of the food consumed. So, the true digestibility coefficient of the protein has been amounted to about 100%  and the amount of food ingested by the adult fish has been about 100g.  Therefore, amongst 100% protein availability in the consumed food the metabolic protein has been 3.6 g, digestible protein is 96.4 g while the apparent digestible coefficient is about 96.4%. When the adult fish sample is being provided rice powder as feed at different quantities  the digestibility coefficient % also found to be varied. When feeding level has been 2 g/fish/day  the nutrient composition has been 86.2% protein, 87.66% lipids, 86.78 % fibres and 88.12% nitrogen free extracts as well. Similarly, the digestibility coefficient % varies in relation to the variations of nutrient composition.