The Effect of Sirna Interference on CDC14A Expression in the Gastric Cancer Cell Line MNK-45

Abstract

Gastric cancer is a malignant tumor that seriously threatens the health of the nation as a whole. However, there is still a lack of precise treatment for advanced and metastatic cancer patients who cannot undergo surgery. Currently, tumor molecular targeted biological therapy has shown potential in the treatment of advanced gastric cancer. The cell cycle 14A (CDC14A) phosphatase is a dual specific phosphatase that widely regulates cell division in eukaryotes. CDC14A can inhibit cell division by mediating the G2/M phase transition, and dysregulation of CDC14A expression plays a role in carcinogenesis. Studies have shown that CDC14A may affect the development of highly microsatellite unstable (MSI-H) gastric cancer by deactivating phosphatase function, but the mechanism by which abnormal expression of CDC14A affects gastric cancer development is not yet clear. Therefore, we investigated the effect of CDC14A on gastric cancer cells by upregulating and knocking down the expression of CDC14A via siRNA. The results revealed that downregulation of CDC14A expression promoted cell entry into the G1 phase, inhibited cell proliferation and in vitro colony formation, whereas upregulation of CDC14A expression promoted cell colony formation. This study preliminarily confirms that CDC14A affects the development of gastric cancer by interfering with cell division and proliferation, laying the foundation for further revealing the mechanism of action of CDC14A in gastric cancer and providing new strategies for molecular targeted biological therapy of gastric cancer.