Identification and Diagnosis of Possible Mutations in Human P-53 Lymphocytes

Abstract

In this study, some diagnosed cases of chronic lymphocytic leukemia (CLL) with possible mutations in the P-53 gene in the P53 gene deletion in human lymphoma genome human B lymphocyte genome were investigated. The ELISA technique was used to determine the frequency of p-53 protein expression in 20 patients diagnosed with CLL being analyzed in stages II-III / IV.

The average concentrations of p-53 proteins in 17 of the 20 cases were found to be 16.76 μg / dl, with CV = 0.5% and the probability index p = 0.034. It was found that the percentage of p-53 positive isoform proteins increased above normal with disease progression: 15% ± 2 in stages 1-2, compared to 100% in stages 3-4.  In many studies in recent years, p-53 protein has been shown to mediate autophagy-inducing genes and stimulate autophagy by inhibiting protein kinase B, AMPK protein, and the mTOR complex. The role of p-53 protein in the autophagy of cancer cells could be used to develop a new anti-cancer therapeutic approach.

ELISA method has proven to be a useful prognostic tool for CLL for the application of personalized treatment in cases diagnosed with CLL resistance to the specific treatment applied by the first line.